Browsing by Author "Nancy A. Monteiro-Riviere, Committee Chair"

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    Effects of Mechanical Stimuli on Biological Interactions with Amino Acid-Derivatized Fullerenes at the Tissue and Cellular Levels
    (2007-07-05) Rouse, Jillian Grace; Peter L. Mente, Committee Member; Elizabeth G. Loboa, Committee Co-Chair; Nancy A. Monteiro-Riviere, Committee Chair
    Engineered nanomaterials have structural features with at least one dimension in the 1—100 nm range. Because of their small size, nanoparticles possess unique chemical, mechanical, electrical, optical, magnetic, and biological properties that make them ideal candidates for a variety of novel commercial and medical applications. Particularly, carbon-based nanomaterials such as fullerenes, nanotubes, and nanowires are considered key elements in the development of new nano-applications with the potential to be used in everything from biomedicine and drug delivery systems to nanoelectronics and energy conservation mechanisms. Relatively unknown, however, is how exposure to nanoscale particles effects normal biological functions and processes. A major focus of recent toxicological research has begun to investigate the interactions between the biological environment and engineered nanoparticles and to determine appropriate safety standards that should be considered when interacting with nanomaterials. The purpose of this research is to investigate how fullerene-based amino acids interact with the biological environment both at the tissue and cellular levels and to identify factors, such as mechanical stimulation, that increase these interactions.
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    Toxicity of Nanomaterials and Mechanisms of Endocytic Pathways
    (2009-12-08) Zhang, Leshuai; Andrew R. Barron, Committee Member; Xinrui Xia, Committee Member; Roger Narayan, Committee Member; Jim E. Riviere, Committee Member; Nancy A. Monteiro-Riviere, Committee Chair
    Engineered nanoparticles (NP) are small in size, large in surface area and can have different types of coating and functionalization. In addition, there are other characteristics of the NP such as unique chemical, mechanical, electrical, optical, and magnetic properties, as well as ability of tracking and quantification provide the possibility to utilize them in NP-based diagnosis or therapy. The potential for NP use in commercialized biomedical applications is increasing but the toxicity and biodistribution in biological systems is unknown. Since the 1990’s, a focus of NP research has been to uncover the associations between NP interactions in vitro and in vivo. However, NP interactions with skin has been limited. The goal of this research is to investigate the effects of several types of NP of different sizes, charges, and surface coatings on skin, or in human epidermal keratinocytes (HEK). Quantum dots (QD) have received attention due to their fluorescent properties. The penetration and interaction of QD in skin and the effect on HEK were studied. QD with different surface coatings remained in the stratum corneum layers or in the outer root sheath of hair follicles of rat skin and porcine skin. However, QD showed an increase in penetration in the dermis of the abraded skin but not tape-stripped rat skin. HEK viability decreased and cytokine release increased with QD. QD were internalized by HEK and localized freely or in cytoplasmic vacuoles. We investigated the interaction and uptake of carbon based NP such as multi-walled carbon nanotubes, amino acid derived single-walled carbon nanotubes, and fullerene functionalized peptides in HEK. The uptakes of NP were shown and the mechanism of how NP were incorporated into cells was also investigated. Carboxylic acid coated QD or fullerene peptides were utilized as targets to explore the endocytic mechanisms. These studies suggested that general pathways such as caveolae/lipid rafts, as well as specific receptors such as G protein coupled receptor and low density lipoprotein/scavenger receptors can regulate NP uptake in cells.