Binding and Local Dynamics of Iso-1-cytochrome c to Substrate-Supported Lipid Nanotube Arrays

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Title: Binding and Local Dynamics of Iso-1-cytochrome c to Substrate-Supported Lipid Nanotube Arrays
Author: Smith, Shani Jomella
Advisors: Edmond Bowden, Committee Member
Alex Smirnov, Committee Chair
Tatyana Smirnova, Committee Member
Abstract: A study of the binding and local dynamics of iso-1-cytochrome c to lipid nanotube arrays composed of 1,2-Diheptadecanoyl-sn-Glycero-3-[Phospho-rac-(1-glycerol)] (DOPG), an anionic phospholipid on an nanoporous anodic aluminum oxide (AAO) substrate was undertaken to gain an understanding of the effects of nanoscale confinement on protein binding. Iso-1-cytochrome c was spin-labeled with 1-oxyl-2,2,3,3-tetramethylpyrolline-3-methylmethanesulfonate (MTSL) at cysteine 102. The binding and local dynamics of the spin-labeled iso-1-cytochrome c bound to multilamellar liposomes was compared with the binding to lipid nanotube arrays. Location of spin-labeled cysteine 102 of iso-1-cytochrome c with respect to the bilayer was probed using an EPR oxygen accessibility experiment. CD monitored changes in the heme coordination in iso-1-cytochrome c upon binding to DOPG.
Date: 2004-01-15
Degree: MS
Discipline: Chemistry
URI: http://www.lib.ncsu.edu/resolver/1840.16/108


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