Binding and Local Dynamics of Iso-1-cytochrome c to Substrate-Supported Lipid Nanotube Arrays
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Date
2004-01-15
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Abstract
A study of the binding and local dynamics of iso-1-cytochrome c to lipid nanotube arrays composed of 1,2-Diheptadecanoyl-sn-Glycero-3-[Phospho-rac-(1-glycerol)] (DOPG), an anionic phospholipid on an nanoporous anodic aluminum oxide (AAO) substrate was undertaken to gain an understanding of the effects of nanoscale confinement on protein binding. Iso-1-cytochrome c was spin-labeled with 1-oxyl-2,2,3,3-tetramethylpyrolline-3-methylmethanesulfonate (MTSL) at cysteine 102. The binding and local dynamics of the spin-labeled iso-1-cytochrome c bound to multilamellar liposomes was compared with the binding to lipid nanotube arrays. Location of spin-labeled cysteine 102 of iso-1-cytochrome c with respect to the bilayer was probed using an EPR oxygen accessibility experiment. CD monitored changes in the heme coordination in iso-1-cytochrome c upon binding to DOPG.
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spin-labeling EPR
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Degree
MS
Discipline
Chemistry
