Development of a Genetic Linkage Map in Abies nordmanniana Accession 9M

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Title: Development of a Genetic Linkage Map in Abies nordmanniana Accession 9M
Author: Hudson, Emily Elizabeth
Advisors: D. Michael Benson, Committee Member
Henry V. Amerson, Committee Co-Chair
L. John Frampton, Committee Co-Chair
Abstract: Genetic linkage maps have been constructed for many species of conifers; however, none has been previously published for an Abies species. A genetic linkage map of Nordmann fir (Abies nordmanniana (Steven) Spach) open-pollinated family 9M was constructed using AFLP and RAPD markers developed from megagametophytic DNA. In all, 556 markers were grouped at LOD ≥ 5.0, θ = 0.30, into 19 linkage groups that covered 1977 cM (Kosambi). Framework maps were ordered with interval support ≥ 3.0 for each linkage group. Accessory markers were attached to the nearest framework marker based on LOD scores and recombination fractions. Significant linkage distortion (approximately 10% of the 556 markers based on a chi-square test at p ≤ 0.05) from the expected 1:1 Mendelian segregation ratio was recognized. The genome size was estimated to be 2471 cM, and this map provided 80% coverage of the genome. Attempts to map a trait locus (based on disease phenotype in an inoculated mapping population) for disease resistance to Phytophthora cinnamomi Rands were unsuccessful. This Abies linkage map will be important to the Christmas tree industry for marker-assisted selection of useful traits such as pest resistance, branching characteristics, height, growth rate, and post-harvest needle retention.
Date: 2005-06-15
Degree: MS
Discipline: Forestry

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