Characterization of the Methanococcus jannaschii RNase P holoenzyme.

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dc.contributor.advisor J.W. Brown, Chair en_US
dc.contributor.advisor L. Parks, Member en_US
dc.contributor.advisor P. Wollenzien, Member en_US
dc.contributor.author Andrews, Andrew J. en_US
dc.date.accessioned 2010-04-02T18:04:57Z
dc.date.available 2010-04-02T18:04:57Z
dc.date.issued 2000-08-10 en_US
dc.identifier.other etd-20000810-110103 en_US
dc.identifier.uri http://www.lib.ncsu.edu/resolver/1840.16/1540
dc.description.abstract The RNase P RNAs from Methanococcus and Archaeoglobus lack secondary structural features essential for the recognition of pre-tRNA. In the absence of protein, these RNase P RNAs lack catalytic activity. How the Methanococcus and Archaeoglobus RNase P holoenzymes compensate for the absent RNA structural elements is not known, but we hypothesize that it is via additional proteins. The Methanococcus jannaschii RNase P holoenzyme has been purified for structural and functional characterization. This enzyme has a buoyant density in Cs2SO4 of 1.39 g/ml and an apparent molecular weight of greater than 400kDa. The holoenzyme has a Km of 68 nM, a kcat of 37nM min-1 (similar to that of other RNase P enzymes) and tolerates a wide range of ionic conditions. Six potential protein subunits have been identified on the basis of copurification with enzymatic activity. The molecular weight of three of these bands is consistent with apparent holomologs of RNase P proteins from Methanobacterium thermoautotrophicum and Saccharomyces cerevisiae. en_US
dc.rights I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. en_US
dc.title Characterization of the Methanococcus jannaschii RNase P holoenzyme. en_US
dc.degree.name MS en_US
dc.degree.level Master's Thesis en_US
dc.degree.discipline Microbiology en_US


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