T Cell Response to Infection by the Porcine Reproductive and Respiratory Syndrome Virus

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dc.contributor.advisor Susan Tonkonogy, Committee Member en_US
dc.contributor.advisor Monte McCaw, Committee Member en_US
dc.contributor.advisor Scott M. Laster, Committee Chair en_US
dc.contributor.advisor Frederick J. Fuller, Committee Member en_US
dc.contributor.author Chen, Hsin-Ying en_US
dc.date.accessioned 2010-04-02T18:05:25Z
dc.date.available 2010-04-02T18:05:25Z
dc.date.issued 2006-01-07 en_US
dc.identifier.other etd-01062005-170050 en_US
dc.identifier.uri http://www.lib.ncsu.edu/resolver/1840.16/1594
dc.description.abstract The purpose of the research has been to characterize the response of naïve or virus-specific swine T lymphocytes from different lymphoid compartments to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Peripheral blood mononuclear cells (PBMCs), tracheobronchial lymph node (TLN) and lateral retropharyngeal lymph node (LLN) cells were labeled with PKH67 green fluorescence dye to measure cell proliferation and surface phenotypes were examined using anti-CD4, anti-CD8 or anti-CD25 monoclonal antibodies. Stimulation with Concanavalin A was also included as a positive control. Our results show that potential virus-specific T lymphocytes were found in the peripheral blood, although no cell proliferation was found in cultures of lymph node cells. We did find that the percentages of CD8+ T cells in cultures of lymph node cells from the virus-infected pig increased after in vitro stimulation with the Powell virus compared to the lymph node cells cultured in media only, suggesting that CD8+ T lymphocytes may play a role in the virus clearance and immune memory to PRRSV. en_US
dc.rights I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. en_US
dc.subject PRRSV en_US
dc.subject T cell response en_US
dc.title T Cell Response to Infection by the Porcine Reproductive and Respiratory Syndrome Virus en_US
dc.degree.name MS en_US
dc.degree.level thesis en_US
dc.degree.discipline Immunology en_US

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