Electrospun Poly(e-caprolactone) (PCL) Nanofibrous Scaffolds for Liver Tissue Engineering

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dc.contributor.advisor Dr. Martin W. King, Committee Chair en_US
dc.contributor.advisor Dr. Marian G. McCord, Committee Member en_US
dc.contributor.advisor Dr. Behnam Pourdeyhimi, Committee Member en_US
dc.contributor.author Gluck, Jessica Marie en_US
dc.date.accessioned 2010-04-02T18:11:48Z
dc.date.available 2010-04-02T18:11:48Z
dc.date.issued 2007-07-18 en_US
dc.identifier.other etd-03222007-145346 en_US
dc.identifier.uri http://www.lib.ncsu.edu/resolver/1840.16/2269
dc.description.abstract Electrospinning is a process utilized to fabricate nanofibers. The latest trend in tissue engineering has been to use these nanofibrous scaffolds for in vitro studies to direct the growth of cellular development. Due to the unique nature of the liver's ability to regenerate itself, this organ has been commonly targeted for tissue engineering purposes. The two main objectives of this study were to electrospin nanofibrous scaffolds from poly(e-caprolactone) (PCL) and also to use these scaffolds for static liver tissue engineering. This two-phase study was divided as such. In order to electrospin PCL the correct solvents and process conditions had to be determined. In accordance with previous literature a solvent mix of chloroform and methanol was used in an initial ratio of 3:1 respectively. The "optimized" electrospinning process conditions were defined as those that consistently produced fibers on a nanoscale and achieved "whipping" at a consistent applied voltage. The electrospinning trials were considered optimized with a plate distance of 12cm, a capillary of ID=0.5mm, capillary exposure of 0.7cm, a flow rate of 0.25mL⁄min, and an applied voltage of 45kV. All samples were collected for a duration of 30 seconds. Upon optimization of the electrospinning process, an investigation was conducted to determine the most appropriate solvent ratio. The solutions examined were at 5% and 10% PCL concentration by weight. Within each concentration five different solvent ratios were examined, ranging from 1:1 to 5:1 chloroform:methanol. As the amount of chloroform increased in the solvent ratio, the viscosity and surface tension of the solutions also increased. A correlation between these physical features of the solutions and the fiber diameter produced existed, and it was determined the "optimal" solution for electrospinning was a 3:1 chloroform:methanol mix and 10% PCL by weight. This solution became the solution used for further electrospinning trials for collection of samples to be used in cell culture. The average fiber diameter produced was 425.83 nm + 185.89 nm. The hepatocyte culture portion of this study was designed to demonstrate the biocompatibility of the PCL nanofibrous scaffolds and to facilitate proliferation in a static cell culture. These tests were conducted in conjunction with the Transplant Lab under Dr. David Gerber's supervision at UNC-CH. The nanofibrous scaffolds when used in hepatocyte culture do not show any significant improvement from the control used. However, it was evident the presence of the PCL and nanofibers encouraged cellular growth. After viability tests, it became clear both the nanofibers and the microfibers from the nonwoven collection fabric directed the growth pattern of the cells. In some cases, cells began to show a growth in 3-D, indicating the early signs of extracellular matrix formation. Using a marker for albumin production, the electrospun scaffolds' cells showed tremendous proliferation and functionality. Overall, PCL can successfully be electrospun using a two solvent mix of 3:1 chloroform to methanol. By varying the solvent ratio and polymer concentration, it is possible to manipulate the fiber diameter and pore size. These scaffolds can be used for tissue engineering and show great promise when used with hepatocytes. en_US
dc.rights I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dis sertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. en_US
dc.subject nanofibers en_US
dc.subject electrospinning en_US
dc.subject tissue engineering en_US
dc.subject polycaprolactone en_US
dc.subject hepatocytes en_US
dc.title Electrospun Poly(e-caprolactone) (PCL) Nanofibrous Scaffolds for Liver Tissue Engineering en_US
dc.degree.name MS en_US
dc.degree.level thesis en_US
dc.degree.discipline Textile Technology Management en_US

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