Comparative Analysis of RNase P

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Title: Comparative Analysis of RNase P
Author: Gantt, John Arthur
Advisors: Dr. Amy M. Grunden, Committee Member
Dr. Stephen J. Libby, Committee Member
Dr. James W. Brown, Committee Chair
Abstract: This Master's Thesis contains a description of the results of two research projects that were worked on over the course of two years. The first was an attempt to heterologously reconstitute the Bacillus subtilis RNase P RNA with the RNase P protein subunits of Methanothermobacter thermoautotrophicus, to identify a functional homology between one or more of the archaeal proteins with the Bacillus subtilis RNase P protein. Unfortunately, the reconstitution experiment could not be completed due to the poor quality of the pre-tRNAasp substrate synthesized. The second project dealt with comparative analysis of bacterial RNase P RNA. Eleven new RNase P RNA genes were amplified, sequenced, and their secondary structures derived by comparative analysis.
Date: 2003-10-01
Degree: MS
Discipline: Microbiology

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