Genetic Analyses of the Federally Endangered Echinacea laevigata using Amplified Fragment Length Polymorphisms (AFLP) - Inferences in Population Genetic Structure and Mating System

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dc.contributor.advisor Qiu-yun (Jenny) Xiang, Committee Chair en_US
dc.contributor.advisor Jon Stucky, Committee Member en_US
dc.contributor.advisor Edward Vargo, Committee Member en_US Peters, Melinda Dean en_US 2010-04-02T18:16:45Z 2010-04-02T18:16:45Z 2006-03-07 en_US
dc.identifier.other etd-10292005-111050 en_US
dc.description.abstract Echinacea laevigata is a federally endangered species and a close relative of the medicinally important E. purpurea. The species has 24 recognized populations restricted to four states (VA, NC, SC, GA). To determine the population structure and outcrossing rate across the range of the species, we conducted AFLP analysis using four primer combinations for 22 populations. The genetic diversity of this species was found to be high based on the level of polymorphic loci (200 of 210 loci; 95.24%) and Nei's gene diversity (ranging from 0.1398 to 0.2606; overall 0.2611). There was significant population genetic differentiation (GST of 0.2941), suggesting possible adaptation to local environments. Results from the AMOVA analysis suggest that a majority of the genetic variance is attributed to variation within populations (70.26%). These results are congruent to a previous allozyme study that examined the genetic makeup of 11 of the 24 populations, excluding any Georgia populations. An isolation by distance (IBD) analysis indicated that genetic differentiation among populations is a function of geographic distance, although long-distance gene dispersal (LDGF) between some populations was suggested based on the NJ tree. An estimate of the outcrossing rate based on genotypes of progenies from six of the 22 populations using a multilocus estimate was 0.833-1.2, where 1.2 is considered complete outcrossing, suggesting that the species is predominantly outcrossing. The remaining populations of E. laevigata have significant levels of population diversity, which is encouraging because the long-term survival of this species will depend on moderate to high levels of genetic diversity and management efforts can focus on other issues instead of increasing the genetic diversity. en_US
dc.rights I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. en_US
dc.subject AFLP en_US
dc.subject outcrossing rate en_US
dc.subject genetic structure en_US
dc.subject Echinacea laevigata en_US
dc.title Genetic Analyses of the Federally Endangered Echinacea laevigata using Amplified Fragment Length Polymorphisms (AFLP) - Inferences in Population Genetic Structure and Mating System en_US MS en_US thesis en_US Botany en_US

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