K. Brevis Cells - Swimming Speeds and Internal Cellular States Over a Range of Temperatures and Light Intensities

Show simple item record

dc.contributor.advisor Howard Glasgow, Committee Member en_US
dc.contributor.advisor JoAnn Burkholder, Committee Member en_US
dc.contributor.advisor Gerald Janowitz, Committee Member en_US
dc.contributor.advisor Daniel Kamykowski, Committee Chair en_US
dc.contributor.author McKay, Laurie Lyn Elizabeth en_US
dc.date.accessioned 2010-04-02T18:18:11Z
dc.date.available 2010-04-02T18:18:11Z
dc.date.issued 2004-03-18 en_US
dc.identifier.other etd-03132004-133747 en_US
dc.identifier.uri http://www.lib.ncsu.edu/resolver/1840.16/2858
dc.description.abstract Karenia brevis is an autotrophic dinoflagellate responsible for many harmful algae bloom (HAB) events in the Gulf of Mexico. Behavior is an integral part of the life history of K. brevis, and swimming data is used in modeling bloom events. In this study, three strains of K. brevis, Apalachicola (APA), Manasota (MAN), and Jacksonville (JAX), are examined under a range of light intensities and temperatures that correspond to the viable range of the organism. In Part I, cell swimming speed is examined over a temperature range from 13∞C - 30∞C and swimming speed is examined with respect to an increasing stimulus light to consider the immediate effect of increasing light on swimming speed. Swimming speed remains fairly constant over all temperatures but the coldest. While examined under increasing light, swimming speed tends to increase. The increase in swimming speed with increases in light is similar to the response of Gyrodinium dorsum, but the increase in speed with Karenia brevis is only observable after increases in light intensity. In Part II, Electron Transfer Rate (ETR) and photosynthetic yield are examined for cultures acclimated to different temperatures. The two strains have opposite trends, APA has higher ETR at higher temperatures and MAN has higher ETR at lower temperatures. In another experiment, in order to examine the relationship between swimming capabilities, light exposure, and the cell's internal physiological state, swimming speed (measured with red light), photosynthetic yield, ETR, Adenosine triphosphate (ATP) concentration, and neutral lipid content are examined after 6 h incubations at 10 light intensities in the radial photosynthetron. ETR increases as light increases from low to higher light and swimming speed decreases. As light increases more, ETR decreases and swimming speed increases. With further increases in light, ETR and swimming speed decrease. Neutral lipids follow a pattern similar to ETR, only lipids peak after ETR at a light intensity that corresponds to the increase in swimming speed. The results, which suggest complex swimming-physiological relationships, generate questions to how cells partition energy. Swimming speed, yield, and ETR are examined over 12 h incubations as well - with sampling throughout the day. The patterns in these experiments are more difficult to interpret. In Chapter 2, cell surface aggregation patterns among K. brevis strains are examined. The strains show distinct and consistent surface patterns. There are no apparent differences in lipid content and organelle distribution in samples of the aggregates of the strains. Throughout this study, APA tends to swim the fastest when taped in the dark, whereas JAX tends to swim the slowest. Despite variation among the strains examined, the overall swimming speed of the species is in line with accepted values. Information from the three strains is combined to produce a species response to light and temperature ranges, and for comparison with physiological data. en_US
dc.rights I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. en_US
dc.subject swimming speed en_US
dc.subject dinoflagellate en_US
dc.subject physiology en_US
dc.subject bioconvection en_US
dc.subject gyrotaxis en_US
dc.subject behavior en_US
dc.title K. Brevis Cells - Swimming Speeds and Internal Cellular States Over a Range of Temperatures and Light Intensities en_US
dc.degree.name MS en_US
dc.degree.level thesis en_US
dc.degree.discipline Marine, Earth and Atmospheric Sciences en_US


Files in this item

Files Size Format View
APA50.AVI 2.984Mb Unknown View/Open
APA631.AVI 2.532Mb Unknown View/Open
APA1660.AVI 3.694Mb Unknown View/Open
JAX50.AVI 3.664Mb Unknown View/Open
etd.pdf 28.91Mb PDF View/Open
JAX1660.AVI 4.021Mb Unknown View/Open
MAN66.AVI 4.077Mb Unknown View/Open
MAN2159.AVI 2.469Mb Unknown View/Open

This item appears in the following Collection(s)

Show simple item record