Recombinant Lactobacillus as a Vaccine Vector for HIV

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dc.contributor.advisor Dr Fred J Fuller, Committee Member en_US
dc.contributor.advisor Dr Herman F Staats, Committee Member en_US
dc.contributor.advisor Dr Gregg A Dean, Committee Chair en_US
dc.contributor.advisor Dr Todd R Klaenhammer, Committee Member en_US Kakarla, Sudha en_US 2010-04-02T18:19:02Z 2010-04-02T18:19:02Z 2006-08-21 en_US
dc.identifier.other etd-08142006-204727 en_US
dc.description.abstract The objective of the research was to evaluate the efficacy of Lactobacillus as a vaccine vector against human immunodeficiency virus (HIV) infection. Lactobacillus gasseri, a well characterized commensal of the human intestinal tract, was engineered to express HIV antigens. In this study we have genetically cloned the HIV gag gene into Lactobacillus gasseri, confirmed the expression of Gag protein and evaluated the immunogenicity of the recombinant bacteria in a murine model. To evaluate the immunogenic efficacy of the novel vaccine we have assayed the serum levels of IgG and also mucosal IgA levels (fecal and vaginal washes). Antigen-specific cell mediated immune responses were assessed using IFN-γ ELISPOT, IL-2 ELISPOT and flow cytometric quantification of p24 Gag peptide AMQMLKETI specific CD8+ CD3+ T cells. We also evaluated a prime-boost vaccination strategy where mice were primed with a recombinant p24 Gag intradermally with adjuvant (CpG oligonucleotides) and then boosted orally with recombinant Lactobacillus expressing Gag. The results indicate that prime-boost strategy efficiently elicited anti p24Gag serum IgG levels and mucosal cell mediated immune responses whereas Lactobacillus expressing Gag administered alone was efficient in generating cell mediated immune responses in the intestinal mucosa. Hence lactobacilli have the potential to be a mucosal vaccine delivery vehicle for HIV antigens. en_US
dc.rights I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. en_US
dc.subject mice en_US
dc.subject vaccine vector en_US
dc.subject gag gene en_US
dc.subject HIV en_US
dc.subject Lactobacillus en_US
dc.title Recombinant Lactobacillus as a Vaccine Vector for HIV en_US MS en_US thesis en_US Immunology en_US

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