Geminivirus Movement Protein Interactions with Host Proteins in the Yeast Two-Hybrid System

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dc.contributor.advisor Dr. I.T.D. Petty, Committee Chair en_US Aylor, William Wesley en_US 2010-04-02T18:19:03Z 2010-04-02T18:19:03Z 2002-08-26 en_US
dc.identifier.other etd-08122002-205507 en_US
dc.description.abstract Geminiviruses utilize two movement proteins in cell to cell movement within their plant hosts. The proteins, BR1 and BL1, enable the virus to move from the host nucleus into the cytoplasm and then through the plasmodesmata into a neighboring cell. The BR1 protein attaches to the viral DNA in the nucleus and moves the complex into the cytoplasm where BL1 interacts to complete the movement. Besides cell to cell movement, geminiviruses may also move systemically throughout the plant. In geminiviruses that are not well adapted to a given host the coat protein, AR1, is needed for systemic movement. Viral interactions with host proteins are vital for processes such as replication, however, little is known about virus-host interactions involved in intercellular movement. The yeast-two-hybrid system enables the detection of protein-protein interactions based on selective yeast growth. Interactions between a viral bait and host prey allow the growth of yeast on nutrient deficient plates. The viral baits were derived from geminiviruses bean golden mosaic virus (BGMV) and squash leaf curl virus (SLCV). BGMV BR1, BGMV AR1, and SLCV BR1 were screened against a cDNA library from the plant host Nicotiana benthamiana. Possible relevant interacting host proteins that allowed growth when co-expressed with one or more of these viral baits and not when co-expressed with irrelevant baits have been isolated and identified. In addition to screening a cDNA library of a plant host, the movement proteins and the coat protein were tested against each other for interactions using the yeast two-hybrid system. en_US
dc.rights I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. en_US
dc.subject geminivirus en_US
dc.subject yeast two-hybrid en_US
dc.title Geminivirus Movement Protein Interactions with Host Proteins in the Yeast Two-Hybrid System en_US MS en_US thesis en_US Microbiology en_US

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