Embryonic Epithelial Development is Modulated by Extracellular Calcium
No Thumbnail Available
Files
Date
2002-09-23
Authors
Journal Title
Series/Report No.
Journal ISSN
Volume Title
Publisher
Abstract
This study investigated the effects of calcium on epithelial differentiation in organ-cultured embryonic chick duodenum. Alteration of calcium ion concentration of culture medium above or below the physiological Ca2+ concentration of embryonic chick serum strongly influenced gastrointestinal development. Culture of 16- or 18-day embryonic intestine in high Ca2+ resulted in elevated activity of alkaline phosphatase, a brush border digestive enzyme, and an increase in goblet cell number within the epithelium. Conversely, low Ca2+ reduced both indices of functional differentiation. Lead acetate, a disruptor of Ca2+ homeostasis, was as effective as low Ca2+ in impeding epithelial differentiation. The voltage sensitive Ca2+-channel (VSCC) antagonists verapamil and nifedipine were used to selectively block Ca2+ influx into the cultured tissue. Both verapamil, a non-specific channel blocker, and nifedipine, which is highly selective for L-type VSCCs, significantly inhibited differentiation, but nifedipine gave more consistent results. Imaging of calcium within the epithelium of cultures was performed by confocal scanning laser microscopy, using the fluorescent calcium probe indo-1. Quantification of Ca2+ within enterocytes revealed that both verapamil and nifedipine reduced intracellular Ca2+ concentration. Together, these results indicate that extracellular Ca2+ influences epithelial differentiation by modulating intracellular Ca2+-dependent signaling pathways, possibly mediated by VSCCs.
Description
Keywords
calcium, regulation, extracellular, embryo, epithelium
Citation
Degree
PhD
Discipline
Physiology