Antimicrobial Polypeptides, Piscidins and Histone-Like Proteins, in Important Aquacultured Fish and the Effect of Nutrition on their Expression and Susceptibility to Infection in Hybrid Striped (Sunshine) Bass (Morone saxatilis male x M. chrysops female)

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dc.contributor.advisor Gregory A. Lewbart, Committee Member en_US
dc.contributor.advisor Edward J. Noga, Committee Chair en_US
dc.contributor.advisor Russell J. Borski, Committee Member en_US
dc.contributor.advisor William L. Gordon, Committee Member en_US
dc.contributor.advisor R. Wayne Litaker, Committee Member en_US
dc.contributor.author Corrales, Jone en_US
dc.date.accessioned 2010-04-02T18:31:34Z
dc.date.available 2010-04-02T18:31:34Z
dc.date.issued 2008-05-12 en_US
dc.identifier.other etd-05052008-171103 en_US
dc.identifier.uri http://www.lib.ncsu.edu/resolver/1840.16/3541
dc.description.abstract Innate immunity is the initial response of a host in defending against pathogen invasion (Beutler 2004, Janeway and Medzhitov 2002). The key features of an innate immune response are that it is: non-specific (broad-spectrum), rapid (often within minutes to hours) and conserved in its pattern of recognition. Antimicrobial polypeptides (AMPP) are a key component of the innate system and present in virtually all life forms (Zasloff 2002). One of the most widespread AMPP in fish are the piscidins. Piscidins have potent, broad-spectrum activity against viruses (Chinchar et al 2004), bacteria (Silphaduang and Noga 2001), fungi (Lauth et al 2002) and parasites (Colorni et al, Accepted). Another family of AMPP are histone-like proteins (HLPs), which are highly homologous to core nuclear histones (Robinette et al 1998, Noga et al 2002). Because disease is often the major cause of economic losses in fish farming, understanding how stress affects innate immunity (e.g., expression of AMPP) and thus disease susceptibility could be highly beneficial to the industry. One very important factor that can contribute to stress in aquaculture is inadequate nutrition. Feed is the single largest expenditure in semi-intensive and intensive farms (Lunger et al 2007, Twibell et al 2003, Mbahinzireki et al 2001). Thus, reducing feed costs while still maintaining optimal health is critical for successful production. As nutrient intake decreases, metabolic processes are impaired and susceptibility to disease increases. I anticipated that this would be reflected in the levels of piscidins and HLPs. The overall goal was to investigate the levels of these antibiotics that indicate fish are stressed prior to any pathological signs. For this first, an ELISA for piscidin 4 was developed and validated and its application to asses stress was confirmed. Using this ELISA and various other independent assays the presence of piscidin 4 related AMPP were detected in important aquacultured fish. Next, I found that nutritional deprivation has a deleterious effect on piscidin 4 expression and that susceptibility to an ich increases in hybrid striped bass (Morone saxatilis ♂x M. chrysops ♂). The nutritional deprivation also lead to dermatological condition in channel catfish. en_US
dc.rights I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dis sertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. en_US
dc.subject piscidin en_US
dc.subject histone-like proteins en_US
dc.subject ELISA development en_US
dc.subject aquacultured fish en_US
dc.subject striped bass en_US
dc.subject white bass en_US
dc.subject European seabass en_US
dc.subject red drum en_US
dc.subject compensatory growth en_US
dc.subject feed rate en_US
dc.subject age en_US
dc.subject size en_US
dc.subject ich challenge en_US
dc.subject susceptibility to infection en_US
dc.title Antimicrobial Polypeptides, Piscidins and Histone-Like Proteins, in Important Aquacultured Fish and the Effect of Nutrition on their Expression and Susceptibility to Infection in Hybrid Striped (Sunshine) Bass (Morone saxatilis male x M. chrysops female) en_US
dc.degree.name PhD en_US
dc.degree.level dissertation en_US
dc.degree.discipline Comparative Biomedical Sciences en_US


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