A Genomic-Based Search for Novel Soybean (Glycine max L.) Allergens

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dc.contributor.advisor William F. thompson, Committee Member en_US
dc.contributor.advisor Bryon Sosinski, Committee Member en_US
dc.contributor.advisor Wesley Burks, Committee Member en_US
dc.contributor.advisor Steven L. Spiker, Committee Member en_US
dc.contributor.advisor Arthur Weissinger, Committee Chair en_US
dc.contributor.author Riascos, John Jaime en_US
dc.date.accessioned 2010-04-02T19:08:22Z
dc.date.available 2010-04-02T19:08:22Z
dc.date.issued 2009-04-05 en_US
dc.identifier.other etd-12172008-120356 en_US
dc.identifier.uri http://www.lib.ncsu.edu/resolver/1840.16/5129
dc.description.abstract Allergic reactions to legumes range from mild skin rashes to life-threatening systemic anaphylaxis. These reactions are caused by naturally occurring proteins in the plant that upon interaction with the immune system of allergic patients, trigger the production allergen-specific IgE antibodies. Soybean is considered one of the eight most allergenic foods and, along with peanut, are the most important legumes associated with food allergies. Currently, the only method to manage soybean allergy is to remove it from the diet. However, this has proven virtually impossible for the majority of soy-allergic patients because soy products are "hidden" in many processed foods. Despite the fact that several soybean allergens have already been characterized, the goal of this investigation was to identify and characterize novel soy allergens, since these data might be used in the development of therapeutic agents to treat soy-allergic patients. Further, because recent investigations have demonstrated the potential use of soybean protein to desensitize people with more severe allergy to foods such as peanut, the identification of novel soy allergens could also advance the development of this desensitization strategy. Because plant food allergens belong to a small number of protein families present in a wide array of species, we hypothesized that soy proteins that are orthologs of proteins known to be allergenic in other crop species could also be allergens in soy. In this investigation we constructed a cDNA library from developing soy seeds and isolated four full-length cDNAs encoding novel candidate allergens. The proteins these cDNA encoded were expressed in a bacterial system and were analyzed by a combination of genomic, proteomic and immunological techniques. The results presented here demonstrate that we have characterized a novel soybean allergen, the seed specific biotinylated protein (SBP). Our results also indicate that SBP is potentially a major allergen because it exhibits reactivity with the majority of soy-allergic patient sera tested in the study. Further characterization of this protein has led us to hypothesize that the peanut ortholog of soy SBP is also allergenic. The soybean SBP is thus an important allergen and should be considered in the diagnosis and treatment of soybean allergy. en_US
dc.rights I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dis sertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. en_US
dc.subject Allergen en_US
dc.subject Peanut en_US
dc.subject Soybean en_US
dc.subject cDNA library en_US
dc.title A Genomic-Based Search for Novel Soybean (Glycine max L.) Allergens en_US
dc.degree.name PhD en_US
dc.degree.level dissertation en_US
dc.degree.discipline Crop Science en_US

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