Fundamental Study and Method Development for Surface-based Laser Desorption Ionization Imaging Mass Spectrometry

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dc.contributor.advisor David C. Muddiman, Committee Member en_US
dc.contributor.advisor Edmond F. Bowden, Committee Member en_US
dc.contributor.advisor Lin He, Committee Chair en_US
dc.contributor.advisor Morteza G. Khaledi, Committee Member en_US
dc.contributor.author Liu, Qiang en_US
dc.date.accessioned 2010-08-19T18:14:20Z
dc.date.available 2010-08-19T18:14:20Z
dc.date.issued 2010-05-01 en_US
dc.identifier.other etd-02262009-143514 en_US
dc.identifier.uri http://www.lib.ncsu.edu/resolver/1840.16/6174
dc.description.abstract By providing both the chemical identity and the spatial organization of each component in biological samples, Imaging Mass Spectrometry (IMS) becomes an emerging tool in clinic and pharmacological study. Most work in IMS has been focused on protein and peptide mapping in biological samples to take advantage of effective analyte ionization in MALDI-MS, and also partially due to the limitation of MALDI-MS in small molecule detection. The focus of my research is to develop novel tools to image spatial distribution of small molecules in biological samples. A surface-based mass spectrometric imaging method, i.e. Desorption/Ionization on Silicon (DIOS), was used for biological surface analysis in the concept-proof investigation. More over, possible proton transferring pathways and impact of local chemical environment have been systematically investigated in the fundamental understanding of ionization mechanism of SALDI-MS. Based on the finding on the SALDI mechanism, a hybrid ionization approach, ME-SALDI has been developed by combing the strength of the conventional MALDI matrix and SALDI, where the improved detection sensitivity with reduced matrix-analyte interference and the improved imaging capability through analysis of mouse brain and heart sections has been demonstrated. In addition, the impact of vacuum stability of matrix in ME-SALDI-IMS applications has been examined. A solvent free, homogenous and reproducible sublimation method has been developed for ionic matrix in ME-SALDI, by which improved vacuum stability and MS detection have been achieved. Furthermore, a new generation of meso-porous oxide substrate was developed as a novel ME-SALDI substrate with a superior storage stability, extended detectable mass range and robust substrate preparation. en_US
dc.rights I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dis sertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. en_US
dc.subject machaniam en_US
dc.subject metabolite en_US
dc.subject mass spectrometry en_US
dc.subject imaging mass spectrometry en_US
dc.subject MS en_US
dc.title Fundamental Study and Method Development for Surface-based Laser Desorption Ionization Imaging Mass Spectrometry en_US
dc.degree.name PhD en_US
dc.degree.level dissertation en_US
dc.degree.discipline Chemistry en_US


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