Efficacy of Immersion and Intraperitoneal Vaccination Against Yersinia Ruckeri and the Effects on Immune Response in Rainbow Trout, Oncorhynchus Mykiss

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Title: Efficacy of Immersion and Intraperitoneal Vaccination Against Yersinia Ruckeri and the Effects on Immune Response in Rainbow Trout, Oncorhynchus Mykiss
Author: Jenkins, Thomas Burton II
Advisors: Dr. David A. Dickey, Committee Member
Dr. Michael G. Levy, Committee Member
Dr. Jeffrey M. Hinshaw, Committee Chair
Abstract: In this study, the effectiveness of vaccination against Yersinia ruckeri, using an autogenous bacterin, in rainbow trout (Oncorhynchus mykiss) was assessed. In addition, the immune response detected by enzyme-linked immunosorbent assay (ELISA) was evaluated. Rainbow trout were vaccinated at 25 grams mean body weight by standard bath immersion and intraperitoneal (i.p.) injection (saline-based injection or oil-based adjuvant injection). Bath-challenges occurred on days 42, 101, 181, 286, and 381 post-vaccination. Plasma samples were collected pre-challenge and fourteen days post-challenge for antibody titer analysis. Antibody kinetics were followed prior to first challenge. Relative percent survival (RPS) ranged from 98.96% to 96.85% and did not statistically differ among vaccinated groups. Increased antibody titer was identified in the oil-based adjuvant injection vaccinated group however, statistical significance among vaccinated groups was not determined.
Date: 2003-11-19
Degree: MS
Discipline: Fisheries and Wildlife Sciences
URI: http://www.lib.ncsu.edu/resolver/1840.16/861


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