Expression of Claudin Tight Junction Proteins in Response to Varying Environmental and Physiological Conditions.
| dc.contributor.advisor | Dr. Betty L. Black, Committee Co-Chair | en_US |
| dc.contributor.advisor | Dr. James N. Petitte, Committee Member | en_US |
| dc.contributor.advisor | Dr. Chris M. Ashwell, Committee Member | en_US |
| dc.contributor.advisor | Dr. Brenda J. Grubb, Committee Chair | en_US |
| dc.contributor.advisor | Dr. Russell J. Borski, Committee Member | en_US |
| dc.contributor.author | Ozden, Ozkan | en_US |
| dc.date.accessioned | 2010-04-02T18:27:31Z | |
| dc.date.available | 2010-04-02T18:27:31Z | |
| dc.date.issued | 2009-01-05 | en_US |
| dc.degree.discipline | Zoology | en_US |
| dc.degree.level | dissertation | en_US |
| dc.degree.name | PhD | en_US |
| dc.description.abstract | In this study, the localization and possible functions of claudin tight junction proteins in bird and fish gastrointestinal tract were investigated. Claudin expression was detected in the chick intestine during the last week of embryonic development using microarrays and quantitative real time PCR analyses. Expression profiles differed among the claudins suggesting that they might play different roles in the establishment of intestinal tight junctions during embryonic development. Glucocorticoids are essential for the maturation of numerous tissues during embryonic development and are known to increase the integrity of tight junctions. This study reveals that treatment of 18-day old embryonic intestine with the synthetic glucocorticoid dexamethasone elevates mRNA levels of two claudins within 12 hours. Expression profiles of claudins were also analyzed in the gastrointestinal tract of Mozambique Tilapia. Immunohistochemistry and real-time PCR analyses revealed that various claudins were expressed in the tilapia gut in a tissue specific manner. Salinity transfer experiments indicated that salinity differentially regulates the expression of certain claudins suggesting a role for these proteins in osmoregulation. | en_US |
| dc.identifier.other | etd-12192008-150620 | en_US |
| dc.identifier.uri | http://www.lib.ncsu.edu/resolver/1840.16/3173 | |
| dc.rights | I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dis sertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. | en_US |
| dc.subject | microarrays | en_US |
| dc.subject | tight junctions | en_US |
| dc.subject | claudin | en_US |
| dc.subject | Tilapia | en_US |
| dc.subject | fish | en_US |
| dc.subject | gastrointestinal tract | en_US |
| dc.subject | intestine | en_US |
| dc.subject | glucocorticoid | en_US |
| dc.subject | embryo | en_US |
| dc.subject | chick | en_US |
| dc.title | Expression of Claudin Tight Junction Proteins in Response to Varying Environmental and Physiological Conditions. | en_US |
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