Analysis of Xenopus laevis claudin (Xcla) Tight Junction Genes in Development

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dc.contributor.advisorRobert Grossfeld, Committee Memberen_US
dc.contributor.advisorBrenda Brizuela, Committee Chairen_US
dc.contributor.advisorPat Estes, Committee Memberen_US
dc.contributor.authorXie, Jianzhenen_US
dc.date.accessioned2010-04-02T17:58:03Z
dc.date.available2010-04-02T17:58:03Z
dc.date.issued2005-07-27en_US
dc.degree.disciplineZoologyen_US
dc.degree.levelthesisen_US
dc.degree.nameMSen_US
dc.descriptionNorth Carolina State University Theses Zoology.
dc.descriptionNorth Carolina State University Theses Zoology.
dc.description.abstractEight Xenopus laevis claudin genes, Xcla1, Xcla4B, Xcla5, Xcla6, Xcla12, Xcla16, Xcla18 and Xcla19, were cloned and sequenced. Their normal mRNA expression was determined from cleavage stage to tadpole stage by whole mount in situ hybridization. The protein expression of Xcla5 was detected at the neural stage by whole mount immunostaining. Overexpression of Xcla5 by injection of synthetic mRNA into embryos caused morphological defects similar to those in embryos exposed to Bisphenol A (BPA). Altered patterns of claudin gene expression in the presence of BPA can be correlated with these developmental defects. The results suggest that claudins may play an important role in neural crest cell migration, epithelial-mesenchymal transition and ultimately organogenesis during embryonic development.en_US
dc.formatThesis (M.S.)--North Carolina State University.
dc.formatThesis (M.S.)--North Carolina State University.
dc.identifier.otheretd-04272005-222011en_US
dc.identifier.urihttp://www.lib.ncsu.edu/resolver/1840.16/740
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectclaudinen_US
dc.subjecttight junctionen_US
dc.subjectXenopusen_US
dc.subjectdevelopmenten_US
dc.titleAnalysis of Xenopus laevis claudin (Xcla) Tight Junction Genes in Developmenten_US
dcterms.abstractKeywords: claudin, tight junction, Xenopus, development.
dcterms.abstractKeywords: claudin, tight junction, Xenopus, development.
dcterms.extentx, 62 pages : illustrations (some color)
dcterms.extentx, 62 pages : illustrations (some color)

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