Genetic and Quantitative Analysis of Red Raspberry (Rubus idaeus) for Heat Tolerance and Longer Chilling Requirement

dc.contributor.advisorBryon Sosinski, Committee Co-Chairen_US
dc.contributor.advisorJames Ballington, Committee Memberen_US
dc.contributor.advisorGina Fernandez, Committee Co-Chairen_US
dc.contributor.advisorJames Holland, Committee Memberen_US
dc.contributor.authorMolina Bravo, Ramonen_US Scienceen_US
dc.description.abstractDespite the high level of interest for growing red raspberries (Rubus ideaus) in the southeastern US, production is limited by the lack of adapted, high quality cultivars. Breeding efforts are underway for increasing cultivar availability for this region, however breeding improvements in Rubus are slow and time-consuming. In order to expedite the slow, but effective, breeding process, more molecular breeding tools should be developed. Cultivars adapted to the southeastern US need to tolerate warm summers, and winters with temperature fluctuations. To address this issue, we have developed a genetic linkage mapping population from a cross that segregates for the tolerance of both conditions, (R. parvifolius × ‘Tulameen’) × ‘Qualicum’. This population was used for the construction of a genetic linkage map and for quantitative trait loci (QTL) analysis of heat tolerance, and chilling requirement for tolerance to fluctuating winter temperatures. As expected, seven linkage groups were created and were similar to the already published map. Because heat tolerance is a difficult trait to measure, a protocol was developed using chlorophyll fluorescence to assess heat tolerance. This protocol was used to measure tolerance in the population, and after QTL analysis, 3 QTL explained ~35% of the variation. Chilling requirements in the population were estimated by measuring bud break under greenhouse conditions. Quantitative analysis was performed on these estimates, and 3 QTL were found in two separate season evaluations, and in most cases co-localization occurred in the same region on the map. These regions explained the majority of the variation in the trait (100-64.5%). In summary, this research has established a protocol that measures heat tolerance in red raspberry, without relying on visual assessment, and has mapped important QTL for further molecular studies. Future research should focus on these regions to develop closely linked molecular markers for marker assisted breeding.en_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dis sertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectheat toleranceen_US
dc.subjectchilling requirementen_US
dc.subjectchlorophyll fluorescenceen_US
dc.subjectgenetic linkage mapen_US
dc.titleGenetic and Quantitative Analysis of Red Raspberry (Rubus idaeus) for Heat Tolerance and Longer Chilling Requirementen_US


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