Functional Genomic Characterization of the Anti-Adipogenic Effects of trans 10, cis 12-Conjugated Linoleic Acid (t10c12-CLA) in a Polygenic Obese Line of Mice

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dc.contributor.advisorEugene J Eisen, Committee Memberen_US
dc.contributor.advisorDavid S Lalush, Committee Memberen_US
dc.contributor.advisorJoseph P Cassady, Committee Memberen_US
dc.contributor.advisorJack Odle, Committee Chairen_US
dc.contributor.authorHouse, Ralph Leeen_US
dc.date.accessioned2010-04-02T18:11:25Z
dc.date.available2010-04-02T18:11:25Z
dc.date.issued2004-07-29en_US
dc.degree.disciplineFunctional Genomicsen_US
dc.degree.levelthesisen_US
dc.degree.nameMSen_US
dc.description.abstractWe analyzed gene expression during t10c12-CLA-induced body fat reduction in a polygenic obese line of mice. Adult mice (N=185) were allotted to a 2x2 factorial experiment consisting of a non-obese (ICR-control) and an obese (M16-selected) line of mice fed a 7% fat, purified diet containing either 1% linoleic acid (LA) or 1% t10c12-CLA. Body weight (BW) gain by day 14 was 12% lower in CLA compared to LA fed mice (P<0.0001). By day 14, t10c12-CLA reduced weights of epididymal, mesenteric and brown adipose tissues as a percentage of BW in both lines by 30, 27 and 58%, respectively, and increased liver weight/BW by 34% (P<0.0001). Total RNA was isolated and pooled (4-5 mice per composite) from epididymal adipose (day 5 &#38; 14) and liver (day 14) of the obese mice to analyze gene expression profiles using Agilent mouse oligo microarray slides (4 per tissue&#8226;day) representing >20,000 genes. Numbers of genes differentially expressed by &#8805; two fold in epididymal adipose (day 5 &#38; 14) and liver (day 14) were 29, 125, and 80, respectively. Of particular interest in adipose, CLA putatively increased expression of the uncoupling proteins (1 and 2), carnitine palmitoyltransferase (L and M), and carnitine translocase, but decreased expression of PPAR-gamma, GLUT-4, perilipin, caveolin-1, adiponectin and resistin (P<0.01). In conclusion, this experiment has revealed candidate genes that will be useful in elucidating mechanisms underlying the potent anti-adipogenic effects of t10c12-CLA.en_US
dc.identifier.otheretd-07292004-115209en_US
dc.identifier.urihttp://www.lib.ncsu.edu/resolver/1840.16/2222
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectapoptosisen_US
dc.subjectmetabolismen_US
dc.subjectde-lipidationen_US
dc.subjectlipiden_US
dc.subjectconjugated linoleic aciden_US
dc.subjectObesityen_US
dc.titleFunctional Genomic Characterization of the Anti-Adipogenic Effects of trans 10, cis 12-Conjugated Linoleic Acid (t10c12-CLA) in a Polygenic Obese Line of Miceen_US

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