Characterization of Complex Protein Mixtures from Human Plasma Fractionation by Capillary Electrophoresis and Mass Spectrometry
| dc.contributor.advisor | Morteza G. Khaledi, Committee Chair | en_US |
| dc.contributor.author | Frantz, Neha Joshi | en_US |
| dc.date.accessioned | 2010-04-02T17:55:24Z | |
| dc.date.available | 2010-04-02T17:55:24Z | |
| dc.date.issued | 2006-08-23 | en_US |
| dc.degree.discipline | Chemistry | en_US |
| dc.degree.level | thesis | en_US |
| dc.degree.name | MS | en_US |
| dc.description.abstract | The purpose of the research has been to develop a simple, quantitative method for characterizing complex protein mixtures using Cohn Fractionation IV-1 Paste as an example. This process step is the starting material for the plasma fractionation process which leads to purified alpha1-antitrypsin, and is an example of a complex protein matrix. Currently, CE analysis of complex plasma samples involves a Borate-NaCl buffer system which does not provide adequate resolution to quantitate protein composition. The goal of this research is to discover optimum CE separation conditions, which would allow rapid, efficient, and inexpensive characterization of these complex samples. A simple, reproducible system utilizing SDS has been developed which provides better resolution and quantitation of complex protein mixtures. This new method is referred to as 'CE-SDS,' and although it is an improvement over the current method, orthogonal methods such as mass spectrometry can provide additional information not captured by this method. | en_US |
| dc.identifier.other | etd-06282006-093358 | en_US |
| dc.identifier.uri | http://www.lib.ncsu.edu/resolver/1840.16/436 | |
| dc.rights | I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. | en_US |
| dc.subject | proteins | en_US |
| dc.subject | capillary electrophoresis | en_US |
| dc.title | Characterization of Complex Protein Mixtures from Human Plasma Fractionation by Capillary Electrophoresis and Mass Spectrometry | en_US |
Files
Original bundle
1 - 1 of 1
