Synthesis and Characterization of a Dye Sensitizing Molecule for Photoelectrochemical DNA Hybridization Detection
| dc.contributor.advisor | Daniel L. Feldheim, Committee Chair | en_US |
| dc.contributor.advisor | Stefan Franzen, Committee Member | en_US |
| dc.contributor.advisor | Charles Boss, Committee Member | en_US |
| dc.contributor.author | Lowe, Lisa Bizzell | en_US |
| dc.date.accessioned | 2010-04-02T18:12:43Z | |
| dc.date.available | 2010-04-02T18:12:43Z | |
| dc.date.issued | 2002-10-28 | en_US |
| dc.degree.discipline | Chemistry | en_US |
| dc.degree.level | thesis | en_US |
| dc.degree.name | MS | en_US |
| dc.description.abstract | A procedure for detection of DNA hybridization is described for the purpose of application in DNA microarray technology. The approach is based on the attachment of a dye-sensitizing molecule to the target oligonucleotide and detection of hybridization of this target to its complementary, surface-attached probe by photoelectrochemistry. A thiolated ruthenium (II) tris(2,2'-bipyridine) dye was synthesized and characterized. The target oligonucleotide was attached to a 10nm gold colloid before hybridization to the probe oligonucleotide, which was immobilized on a polycrystalline gold substrate. Upon hybridization the gold substrate was exposed to the thiolated [Ru(bpy)₃],²⁺ allowing attachment of the molecule to the gold nanoparticles. The photoelectrochemical response was dependent upon applied potential and target concentration. Attachment of the probe oligonucleotide to the gold substrate was investigated, and the electrolyte solution was tested for optimal performance. | en_US |
| dc.identifier.other | etd-07162002-152658 | en_US |
| dc.identifier.uri | http://www.lib.ncsu.edu/resolver/1840.16/2369 | |
| dc.rights | I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. | en_US |
| dc.subject | gene chip | en_US |
| dc.subject | DNA microarray | en_US |
| dc.subject | photoelectrochemistry | en_US |
| dc.subject | ruthenium (II) tris bipyridine | en_US |
| dc.title | Synthesis and Characterization of a Dye Sensitizing Molecule for Photoelectrochemical DNA Hybridization Detection | en_US |
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